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GTP hydrolysis by wild-type and mutant FlhF proteins
| FlhF construct | Km (μM) | Vmax (μmol/min) | kcat (min−1) | kcat/Km (M−1 min−1) |
|---|---|---|---|---|
| Wild type | 36.65 ± 18.01 | 0.42 ± 0.07 | 0.06 ± 0.01 | 1,637 |
| R251G | 9.13 ± 0.90 | 0.50 ± 0.01 | 0.07 ± 0.01 | 7,667 |
| L298R,P299L | 18.04 ± 3.67 | 0.85 ± 0.04 | 0.13 ± 0.01 | 7,206 |
| K222A | 130.70 ± 141.90 | 0.15 ± 0.09 | 0.02 ± 0.01 | 153 |
| D294A | 87.65 ± 95.69 | 0.06 ± 0.03 | 0.01 ± 0.01 | 114 |
