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. 2013 Feb;33(4):739–751. doi: 10.1128/MCB.01264-12

Fig 2.

Fig 2

DCC enhances Trio tyrosine phosphorylation by Fyn in N1E-115 neuroblastoma cells. (A) GFP-Trio was obtained by immunoprecipitation (IP) from N1E-115 neuroblastoma cells transfected with pEGFP-Trio, pRK5-Fyn (WT, wild type; CA, constitutively active), and pRK5-DCC. Phosphotyrosine (pY)-Trio and total Trio were detected by Western blotting using antiphosphotyrosine (4G10) and anti-GFP antibodies, respectively. TCL, total cell lysates. (B) Densitometric analysis of panel A. Error bars indicate SEMs (n = 4; *, P < 0.05). (C) GFP-Trio was obtained by IP from N1E-115 neuroblastoma cells transfected with pEGFP-Trio, pRK5-Fyn, and wild type (WT) or truncated forms of pRK5-DCC, as indicated. Western blotting was conducted as for panel A. TCL, total cell lysates. (D) Densitometric analysis of panel C. Error bars indicate SEMs (n = 4; *, P < 0.05; **, P < 0.005). (E) N1E-115 neuroblastoma cells were transfected with wild-type or truncated forms of pRK5-DCC, as indicated. Neurite outgrowth was assessed by fluorescence microscopy (n = 4; *, P < 0.05; **, P < 0.005).