Fig 8.
Model of Trio regulation during netrin-1/DCC signaling. Netrin-1/DCC engagement induces a Src-dependent phosphorylation of DCC at Tyr1418 (17) and Trio at Tyr2622. TrioY2622 is required for both netrin-1-induced activation of Rac1 and enhanced association with DCC. Phosphorylation of Trio at Tyr2622 participates in maintaining the level of surface DCC at the growth cone plasma membrane leading to axon outgrowth. Therefore, we propose that TrioY2622 is essential for the proper assembly and stability of the DCC/Trio signaling complex at the cell surface of growth cones in order to mediate netrin-1-induced cortical axon outgrowth.