Fig 2.

BAL1 macrodomain 2 is required for recruitment to DNA damage sites. Top, BAL1 protein functional domains, including macrodomains 1 and 2 (orange and red), BBAP binding domain (BBD) (light blue in BAL1) (2), and a region with partial sequence homology to the PARP catalytic domain (dark blue) (domain sizes and amino acids below). GFP-BAL1 constructs are labeled and represented below the BAL1 diagram. Mutations in macrodomain 1 (D126A) and macrodomain 2 (IE 326,327AA [IE-AA]) are shown in black. Representative images of GFP-BAL1 (293T cell) transfectants at baseline and 2 min following laser microirradiation are shown on the right, and the percentages of cells with GFP-BAL1 recruitment to laser-induced DNA breaks are shown on the far right.