Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2013 Feb;33(4):688–700. doi: 10.1128/MCB.00973-12

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2013, American Society for Microbiology. All Rights Reserved.

PMC Copyright notice

Fig 4 — Smk1 is autophosphorylated on residue Y209. (A) Extracts from wild-type SMK1-HA (upper panel) and smk1-K69R-HA (lower panel) strains prepared at the indicated times after transfer to sporulation medium were analyzed by electrophoresis through Phos-tag gels and immunoblotting using an HA antibody. (B) Samples from an smk1Δ/smk1Δ strain harboring the indicated low-copy-number (CEN-based) Smk1-HA plasmids were harvested 9.5 h after meiotic induction and assayed as described for panel A. (C) SMK1/SMK1-HA (WT/WT-HA), smk1-K169R/smk1-K169R-HA (KR/KR-HA), and SMK1/smk1-K169R-HA (WT/KR-HA) cells were harvested 9.5 h following meiotic induction and assayed as described for panel A.