Table 1.
Yeast strains used in this study
| Strain | Genotype | Source or reference |
|---|---|---|
| ALY60 | MATa/MATα ura3/ura3 leu2::hisG/leu2::hisG trp1::hisG/trp1::hisG lys2/lys2 his4/his4 ho::LYS2/ho::LYS2 SMK1-3×HA-KANMX6/SMK1-3×HA-KANMX6 | 20 |
| ALY62 | MATa/MATα ura3/ura3 leu2::hisG/leu2::hisG trp1::hisG/trp1::hisG lys2/lys2 ho::LYS2/ho::LYS2 smk1::LEU2/smk1::LEU2 | 20 |
| LH416 | MATa/MATα ura3/ura3 leu2/leu2 trp1ΔFA/trp1ΔFA lys2/lys2 his3/his3 ho::hisG/ho::hisG SMK1-HIS3MX6/SMK1-3×HA-HIS3MX6 | 16 |
| LRY342 | MATa/MATα ura3/ura3 leu2/leu2 trp1ΔFA/trp1ΔFA lys2/lys2 his3/his3 ho::hisG/ho::hisG smk1-T207A-3×HA-HIS3MX6/smk1-T207A-3×HA-HIS3MX6 | This study |
| LRY343 | MATa/MATα ura3/ura3 leu2/leu2 trp1ΔFA/trp1ΔFA lys2/lys2 his3/his3 ho::hisG/ho::hisG smk1-Y209F-3×HA-HIS3MX6/smk1-Y209F-3×HA-HIS3MX6 | This study |
| LRY344 | MATa/MATα ura3/ura3 leu2/leu2 trp1ΔFA/trp1ΔFA lys2/lys2 his3/his3 ho::hisG/ho::hisG smk1-T207A,Y209F-3×HA-HIS3MX6/smk1-T207A,Y209F-3×HA-HIS3MX6 | This study |
| LRY373 | MATa/MATα ura3-SK1/ura3 leu2::hisG/leu2 trp1::hisG/trp1ΔFA lys2-SK1/lys2 his3/his4 ho::LYS2/ho::hisG smk1-Y209F-3×HA-HIS3MX6/smk1::LEU2 | This study |
| JLY3 | MATa/MATα ura3/ura3 leu2::hisG/leu2::hisG trp1::hisG/trp1::hisG lys2/lys2 ho::LYS2/ho::LYS2 CDC28-43244/CDC28-43244 | 26 |
| JLY4 | MATa/MATα ura3/ura3 leu2::hisG/leu2::hisG trp1::hisG/trp1::hisG lys2/lys2 ho::LYS2/ho::LYS2 CDC28-43244 cak1Δ::TRP1/CDC28-43244 cak1Δ::TRP1 | 26 |
| CMY95 | MATa/MATα ura3/ura3 trp1::hisG/trp1::hisG lys2/lys2 his4/his4 ho::LYS2/ho::LYS2 SSP2-13MYC-KANMX6/SSP2-13MYC-KANMX6 SMK1-3×HA-KANMX6/SMK1-3×HA-KANMX6 | This study |
| CMY58 | MATa/MATα ura3/ura3 leu2::hisG/leu2::hisG trp1::hisG/trp1::hisG lys2/lys2 ho::LYS2/ho::LYS2 ssp2Δ/ssp2Δ | This study |
| LRY274 | MATa/MATα ura3/ura3 leu2/leu2 trp1ΔFA/trp1ΔFA lys2/lys2 his3/his3 ho::hisG/ho::hisG smk1-K69R-3×HA-HIS3MX6/smk1-K69R-3×HA-HIS3MX6 | This study |
| LRY287 | MATa/MATα ura3/ura3 leu2::hisG/leu2 lys2/lys2 trp1-hisG/trpΔFA his3/his4-N ho::LYS2/ho::hisG smk1-K69R-3×HA-HIS3MX6/SMK1 | This study |
| YGS91 | MATa/ ura3 lys2 leu2::hisG trp1::hisG his3 ho::LYS2 ura3::PGPD1-GAL4(848).ER::URA3 PGAL1-NDT80::TRP1 | J. Segall |
| GOY19 | MATa/MATα ura3/ura3 leu2::hisG/leu2::hisG lys2/lys2 trp1::hisG/trp1::hisG his3/HIS3 ho::LYS2/ho::LYS2 SMK1-3×HA-HIS3/SMK1-3×HA-HIS3 SSP2-13MYC-KANMX6/SSP2-13MYC-KANMX6 ura3::PGPD1-GAL4(848).ER::URA3/ura3::PGPD1-GAL4(848).ER::URA3 PGAL1-NDT80::TRP1/PGAL1-NDT80::TRP1 | This study |
| LRY253 | MATa/MATα ura3/ura3 leu2::hisG/leu2::hisG trp1::hisG/trp1::hisG lys2/lys2 his4/his4 ho::LYS2/ho::LYS2 SMK1-HH::LEU2/SMK1-HH::LEU2 | This study |
| PSY3B | MATa leu2::hisG his4-B lys2 ho::LYS2 ura3 can1 | 31 |
| JTY4 | MATa/MATα ura3/ura3 leu2::hisG/leu2::hisG trp1::hisG/trp1::hisG lys2/lys2 his4/his4 ho::LYS2/ho::LYS2 SMK1-HH::LEU2/SMK1-HH::LEU2 ssp2Δ/ssp2Δ | This study |