Fig 2.

(a) Lymphocyte cellularity in thymuses, spleens, and lymph nodes (LN) of Cernu^+/− and Cernu^−/− mice. SEM, standard error of the mean. (b) V(D)J recombination activity in purified CD4⁻ CD8⁻ thymocytes. Transduced cells were identified through expression of the huCD4 gene from the reporter. The % recombination represents the fraction of GFP-positive cells among the huCD4-positive cells. The relative V(D)J activity was calculated by comparison to the level attained in thymocytes from Cernu^+/− mice, used as a 100% activity control. (c) Proliferative capacity of thymocytes. Cernu^−/− Rag1^−/− and Rag1^−/− mice were injected i.p. with anti-CD3.