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(a) Detection of iNKT cells in the spleen by dual staining with anti-TCR-β and CD1d tetramer. (b) Schematic representation of human and murine TCR Vα and Jα clusters, including the locations of TRAV and TRAJ used by iNKT cells. Two Jα segments can be used by murine NKT cells, i.e., TRAV11D and the most downstream segment, TRAV11, because of the duplication of the central TCR Jα segments in mice.
