Fig 1.

Expression and kinase catalytic activity of Map4k4 during skeletal muscle differentiation. (A) RT-PCR analysis of Map4k4 expression in isolated mouse satellite cells versus quadriceps. Data represent means ± SEM for three independent experiments. (B) Expression of Map4k4 during C2C12 myogenic differentiation. Densitometry results are representative of means ± SEM for four independent experiments. (C) C2C12 myoblasts were incubated in DM for the indicated times. Map4k4 kinase activity at each time point was assessed by kinase assay and immunoblotting. Results represent means ± SEM. *, P < 0.05; **, P < 0.01; ***, P < 0.001.