Fig 3.

Map4k4 silencing enhances C2C12 myogenic differentiation. C2C12 myoblasts were transfected with scrambled or Map4k4 siRNA. Cells were transferred to DM 24 h after transfection and differentiated for the indicated times. (A) Expression of myogenic differentiation proteins was assayed by immunoblotting with the indicated antibodies. Data are representative of three independent experiments. (B) Densitometric analysis of the Western blots in panel A. (C) Creatine kinase (CK) activities of transfected cells were measured after 3 days in DM. Data represent means ± SEM for three independent experiments. A.U, absorbance units. *, P < 0.05; ***, P < 0.001.