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Map4k4 silencing increases protein levels of Myf5. (A) C2C12 myoblasts were transfected with scrambled or Map4k4 siRNA and then transferred to DM for the indicated times at 24 h posttransfection. (B) (Top) Myf5 expression determined by immunoblotting. (Bottom) Densitometry of Myf5 normalized to α-tubulin. Data are presented as means ± SEM for three independent experiments. *, P < 0.05; **, P < 0.01.
