Fig 8.

Suppression of Myf5 impairs Map4k4 silencing-enhanced myogenic differentiation. C2C12 myoblasts were transfected with scrambled siRNA or with siRNA against Map4k4, Myf5, or a combination of both. (A) Cells were fixed and immunostained for MyHC (green) and DAPI (blue) at day 3 after differentiation, and myogenic conversion was observed by fluorescence microscopy. Magnification, ×50 (top) and ×100 (bottom). (B) The fusion index was calculated for transfected cells after 3 days in DM. Results represent means and SEM for three independent experiments. ***, P < 0.001. (C) Immunoblots of myogenic differentiation proteins. Data are representative of three independent experiments.