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. 2013 Feb;33(4):712–724. doi: 10.1128/MCB.01226-12

Fig 1.

Fig 1

GBD-Adr1 binds to GAL genes and recruits RNA Pol II in the presence of Bmh1 activity. (A) GBD ChIP. YLL908 (BMH1 bmh2Δ) and YLL1087 (bmh1-ts bmh2Δ) were transformed with a plasmid expressing either GBD (pOBD2) or GBD-Adr1 (154–424) (pGBDA4) under the control of the ADH1 promoter. Triplicate cultures were grown in selective medium plus 2% glucose to maintain the TRP1 GBD plasmids. Samples were processed for a GBD ChIP, and GBD binding to the GAL1-10 and GAL7 promoters relative to the telomere (TEL) was determined by qPCR. (B) RT-qPCR. Triplicate samples for RT-qPCR were collected from the cultures in panel A. GAL1 and GAL7 mRNA levels relative to ACT1 mRNA were determined. (C) RNA Pol II ChIP. Duplicate cultures of the strains listed in panel A were grown in selective medium plus 2% glucose. Samples were collected for RNA Pol II ChIP, and the levels of RNA Pol II binding to the GAL1-10 and GAL7 promoters relative to the telomere were determined by qPCR. The values represent the means and SDs of three biological replicates.