Fig 9.

Interferon-induced caspase-8 upregulation in interferon-sensitive and -resistant cell lines. (A) MDA-MB-321, T47D, and HS578T cells were treated with 1 nM YNS for 3 days following crystal violet staining for cell density evaluation. (B) Caspase-3/7 activity was measured in T47D and HS578T IFN-resistant cell lines following treatment with 1 nM YNS for 48 h. (C) WISH, MDA-MB-321, T47D, BT549T, and HS578T cells were treated with 1 nM YNS for the indicated hours following protein extraction for Western blotting. Error bars represent the standard errors of 2 to 4 independent replicates. The dashed line marks a 3-fold increase in the caspase-8 level. (D) Representative Western blotting for WISH cells treated with 1 nM YNS and/or 1,000 U/ml IFN-γ for the indicated times. (E and F) WISH cells were transfected with siRNA targeting the indicated genes following treatment with 1 nM YNS and/or 1,000 U/ml IFN-γ for 72 h (E) or 24 h (F), after which after cell density (E) or caspase-3/7 activity (F) was monitored. For panels A to C, E, and F, data are represented as means ± standard errors of the means.