Fig 3.

Disruption of pUL29/28 during infection results in elevated expression of a subset of p53-regulatable genes. (A) Fibroblasts were either mock infected or infected with ADwt or ADdel29 at an MOI of 6 and harvested at the indicated times postinfection. Relative RNA was measured by qRT-PCR for the indicated genes and normalized to cellular GAPDH levels. The data are the means for two technical replicates ± standard deviations, with the results being validated in a separate independent biological replicate. Statistical analysis was performed using Student's t test (*, P ≤ 0.05; **, P ≤ 0.01). (B) Experiments were completed as described above, but no statistical difference in expression were observed.