Fig 4.

pUL29/28 influences p21CIP1 and CASP1 expression during infection. (A) Fibroblasts were infected with ADwt or ADdel29 at an MOI of 6 and harvested at the indicated times postinfection. Relative RNA was measured using qRT-PCR. (B) Samples were collected at the indicated times postinfection and processed for Western blot analysis using antibodies against the indicated proteins. (C) For nutrient stress, fibroblasts were serum starved for 24 h and infected with ADwt or ADdel29 at an MOI of 6 in 7% FBS, 25 mM glucose (hi) for 1 h. Cells were then washed and replenished with either normal medium (hi) or medium containing 5.56 mM glucose and no serum (lo). Western blot analysis was performed using whole-cell lysates harvested at the indicated times postinfection as well as from mock-infected samples and antibodies against the indicated proteins.