Fig 6.

pUL29/28 influences p53 and histone H3 K9 acetyl binding to responsive promoters. (A) Fibroblasts were mock infected, treated with 10 μM nutlin-3 for 18 h, or infected with ADwt or ADdel29 at an MOI of 6 for 18 h. Chromatin immunoprecipitation (ChIP) was performed using an antibody against pan-p53 or control IgG. Quantitative PCR was used to determine the relative output DNA levels normalized to input. The data are means from four replicate experiments ± standard deviations and are presented as percentage of input. The PUMA data set represents two experiments. (B) ChIP analysis was performed as described above but using an antibody against histone 3 lysine 9 acetylation. The data are means for four replicates ± standard deviation and are presented as percentages of input. Statistical analysis was performed using Student's t test (*, P ≤ 0.05).