Fig 5.

The G run in intron 2 is necessary for efficient virus replication in nonpermissive cells. (A) CEM-SS, CEM-T4, and CEM-A cells were infected with the NL4-3 virus or mutant derivatives (MOI, 0.01). Virus production was determined by p24 CA capture ELISA of cell-free supernatant collected at the indicated time points. (B) Immunoblot analysis showing A3G expression in CEM-SS, CEM-T4, and CEM-A cells. As a positive control, HEK 293T cells were transiently transfected with a FLAG-tagged A3G expression plasmid. (C) Immunoblot analysis of p24-CA protein in the cell-free supernatant concentrated by sucrose centrifugation at 12 dpi.