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Inactivation of GI2-1 enhances virus replication in PBMCs. (A) PBMCs were infected with the NL4-3 virus or mutant derivatives (MOI, 0.5). Virus production was determined by p24 CA capture ELISA of cell-free supernatant collected at the indicated time points. (B) Immunoblot analysis showing A3G expression in CEM-SS, CEM-T4, and CEM-A cells and PBMCs of two human donors. αTUB, antitubulin antibody. (C) Immunoblot analysis of p24-CA protein in cell-free supernatant at 12 dpi.
