KF11Gag, KK10Gag, and KY9Pol epitopes are presented early after wild-type (WT) HIV-1 infection. Fluorescence-activated cell sorting (FACS) dot plots showing CD107a/MIP1β-expressing HLA-B*57:01-restricted KF11Gag-specific CD8+-gated T cells after coculture at 0, 3, 6, 18, and 24 h with HLA-matched and -mismatched WT HIV-1-infected CD4+ T cells (A) or with KF11 peptide-pulsed HLA-matched or -mismatched CD4+ T cells (B) and plotted on a graph for 5 time points throughout 24 h after infection (C). Activation of KK10Gag-, KY9Pol-, and VL9Vpr-specific CD8+ T cells restricted by HLA-B*27:05 after coculture with HIV-1-infected HLA-matched U937 CD4+ cells or mismatched H9 CD4+ T cells at 0, 3, 6, 18, and 24 h postinfection. (D) Results from one experiment representative of three independent experiments are shown.