Fig 4.

KK10Gag and KY9Pol epitopes are presented in the absence of de novo HIV-1 protein synthesis. Percentage of maximum CD107a/Mip1β expression of KK10Gag-, KY9Pol-, and VL9Vpr-specific CD8⁺ T cells restricted by HLA-B*27:05 after coculture with HIV-1-infected cells in the presence or in the absence of reverse transcriptase inhibition (5 μM AZT). (A) HLA-matched U937 CD4⁺ cells and mismatched H9 CD4⁺ T cells; (B) HLA-matched primary infected CD4⁺ T cells and mismatched primary CD4⁺ T cells. (C and D) Viability of U937 cells and primary CD4⁺ T cells with or without AZT treatment at 24 h postinfection.