Fig 7.

The magnitude of CD8⁺ T cell activation is directly related to the dose of viral antigen. Fluorescence-activated cell sorting (FACS) plots showing CD107a/MIP1β-expressing KK10Gag- and HLA-mismatched (KF11Gag)-specific CD8⁺-gated T cells after coculture at 8 h with U937 CD4⁺ cells infected with of HIV-VLP at a MOI of either 1 or 0.1 (A) and plotted onto a graph for 5 time points throughout the 26 h as mean values and standard errors of the means from triplicates (B). (C) Pie charts showing the simultaneous expression of three effector molecules (IFN-γ, CD107a, and MIP1β) as 3 functions (red), 2 functions (blue), 1 function (green), or nonactivated CD8⁺ T cells (gray) after HIV-VLP infection of B cells (top) or peptide pulsing of B cells at the peptide concentrations indicated above the pie charts (bottom) for KF11Gag-, KK10Gag-, KY9Pol-, and VL9Vpr-specific CD8⁺ T cells.