Table 2.
HIV-specific CD8+ T cell lines and viral vectors used in the study
| Subject ID | HLA restriction allele | Epitope | Protein | Amino acid position in HXB2 | Viral vector | HIV clade B sequence | % CD8+ T cell line specificityb |
|---|---|---|---|---|---|---|---|
| R039 | HLA-B*57:01 | KF11 | Gag | 162–172 | Seq databasea | KAFSPEVIPMF | 98.4 |
| NL4-3 | ----------- | ||||||
| pCMVΔR8.2 | ----------- | ||||||
| R039 | HLA-B*27:05 | KK10 | Gag | 263–272 | Seq database | KRWIILGLNK | 99.6 |
| NL4-3 | ---------- | ||||||
| pCMVΔR8.2 | ---------- | ||||||
| R039 | HLA-B*27:05 | KY9 | Pol | 186–194 | Seq database | KRKGGIGGY | 98.4 |
| NL4-3 | --------- | ||||||
| pCMVΔR8.2 | --------- | ||||||
| R035 | HLA-B*27:05 | VL9 | Vpr | 31–39 | Seq database | VRHFPRIWL | 99.4 |
| NL4-3 | --------- | ||||||
| pCMVΔR8.2 | --------- |
a
Seq database, Los Alamos Immunology database (www.hiv.lanl.gov).
b
Tetramer-positive CD3+CD8+ gated T cells.