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. 2013 Mar;87(5):2721–2734. doi: 10.1128/JVI.02756-12

Table 1.

DNA oligonucleotides used in this work

Plasmid Primer Primer sequence
LTR ex2 ex3 1814 5′ GCG CGC ACG GCA AGA 3′
1817 5′ CTT TAC GAT GCC ATT GGG A 3′
2346 5′ GAA GCG CGC ACG GCA AGA GGC GAG 3′
2347 5′ CGC GAA TTC AGG CCT CTC TC 3′
2381 5′ GGG CTC GAG ACT AGT GGC TGA CTT CCT GGA TG 3′
2384 5′ GGG ACT AGT CAA GAA ATG GAG AAA AAA A 3′
2385 5′ GTA CCC GGG CAC CAA TAA CTG CCT TA 3′
2386 5′ GGG CAT ATG TAT GTT TCA GGG AAA GCT AGG GGA 3′
LTR ex2 ex3 D3down 3817 5′ TTT TCA GAA TCT GCT ATA AGA AAT ACC ATA TTA GGA CGT ATA GTT AGT CCT AGG TGT GAA TAT CAA GCA GGA CAT AAC AAG GTT GGT TCT CTA CAG TAC TTG GCA CTA G 3′
LTR ex2 ex3 ESE −25T>C, −16A>G D3down 3818 5′ TTT TTC AGA ATC TGC TAT AAG AAA TAC CAT ATT AGG ACG TAT AGT TAG TCC TAG GTG CGA ATA TCA GGC AGG ACA TAA CAA GGT TGG TTC TCT ACA GTA CTT GGC ACT AG 3′
LTR ex2 ex3 ESSV D3down 3819 5′ TTT TTC AGA ATC TGC TAT AAG AAA TAC CAT ATT AGG ACG TAT AGT ATC GCC ACG TTG TGA ATA TCA AGC AGG ACA TAA CAA GGT TGG TTC TCT ACA GTA CTT GGC ACT AG 3′
LTR ex2 ex3 ESSV ESE −25T>C, −16A>G D3down 3820 5′ TTT TTC AGA ATC TGC TAT AAG AAA TAC CAT ATT AGG ACG TAT AGT ATC GCC ACG TTG CGA ATA TCA GGC AGG ACA TAA CAA GGT TGG TTC TCT ACA GTA CTT GGC ACT AG 3′
LTR ex2 ex3 D3up 3813 5′ TTT TCA GAA TCT GCT ATA AGA AAT ACC ATA TTA GGA CGT ATA GTT AGT CCT AGG TGT GAA TAT CAA GCA GGA CAT AAC AAG GTA GGT AGT CTA CAG TAC TTG GCA CTA GCA G 3′
LTR ex2 ex3 ESE −25T>C, −16A>G D3up 3814 5′ TTT TTC AGA ATC TGC TAT AAG AAA TAC CAT ATT AGG ACG TAT AGT TAG TCC TAG GTG CGA ATA TCA GGC AGG ACA TAA CAA GGT AGG TAG TCT ACA GTA CTT GGC ACT AGC AG 3′
LTR ex2 ex3 ESSV D3up 3815 5′ TTT TTC AGA ATC TGC TAT AAG AAA TAC CAT ATT AGG ACG TAT AGT ATC GCC ACG TTG TGA ATA TCA AGC AGG ACA TAA CAA GGT AGG TAG TCT ACA GTA CTT GGC ACT AGC AG 3′
LTR ex2 ex3 ESSV ESE −25T>C, −16A>G D3up 3816 5′ TTT TTC AGA ATC TGC TAT AAG AAA TAC CAT ATT AGG ACG TAT AGT ATC GCC ACG TTG CGA ATA TCA GGC AGG ACA TAA CAA GGT AGG TAG TCT ACA GTA CTT GGC ACT AGC AG 3′
LTR ex2 ex3 ESSV D3+1G>C 3889 5′ TTT TTC AGA ATC TGC TAT AAG AAA TAC CAT ATT AGG ACG TAT AGT ATC GCC ACG TTG TGA ATA TCA AGC AGG ACA TAA CAA GCT TGG TTC TCT ACA GTA CTT GGC ACT AG 3′
LTR ex2 ex3 ESSV ESE −25T>C, −16A>G D3 +1G>C 3890 5′ TTT TTC AGA ATC TGC TAT AAG AAA TAC CAT ATT AGG ACG TAT AGT ATC GCC ACG TTG CGA ATA TCA GGC AGG ACA TAA CAA GCT TGG TTC TCT ACA GTA CTT GGC ACT AG 3′
LTR ex2 ex3 ESSV GTV 3885 5′ TTT TTC AGA ATC TGC TAT AAG AAA TAC CAT ATT AGG ACG TAT AGT ATC GCC ACG TTG TGA ATA TCA AGC AGG ACA TAA CCA GCT AAG TAT TCT ACA GTA CTT GGC ACT AGC AG 3′
LTR ex2 ex3 ESSV ESE −25T>C, −16A>G GTV 3886 5′ TTT TTC AGA ATC TGC TAT AAG AAA TAC CAT ATT AGG ACG TAT AGT ATC GCC ACG TTG CGA ATA TCA GGC AGG ACA TAA CCA GCT AAG TAT TCT ACA GTA CTT GGC ACT AGC AG 3′
2588 5′ CTT TAC GAT GCC ATT GGG A 3′
pUCB U1 αD3 3924 5′ GCC CGA AGA TCT CGA TCC TAG CTT GCA GGG GAG ATA CCA TGA TC 3′
3926 5′ TTT TCA CTC GAG CCT CCA CTG TAG 3′
pUCB U1 αD3 (+1G>C) 3925 5′ GCC CGA AGA TCT CGA TCC TAC CTT GCA GGG GAG ATA CCA TGA TC 3′
3926 5′ TTT TCA CTC GAG CCT CCA CTG TAG 3′