Table 2.

Primers used for semiquantitative and quantitative real-time RT-PCR

Viral mRNA type	Primer	Primer sequence
Classes:
E1	1544 (E1)	5′ CTT GAA AGC GAA AGT AAA GC 3′
E7	3392 (E7)	5′ CGT CCC AGA TAA GTG CTA AGG 3′
I4	640 (I4)	5′ CAA TAC TAC TTC TTG TGG GTT GG 3′
E4	3632 (E4)	5′ TGG ATG CTT CCA GGG CTC 3′
All classes	3387	5′ TTG CTC AAT GCC ACA GCC AT 3′
	3388	5′ TTT GAC CAC TTG CCA CCC AT 3′
Unspliced	3389	5′ TTC TTC AGA GCA GAC CAG AGC 3′
	3390	5′ GCT GCC AAA GAG TGA TCT GA 3′
Multiply spliced	3391	5′ TCT ATC AAA GCA ACC CAC CTC 3′
	3392	5′ CGT CCC AGA TAA GTG CTA AGG 3′
vif	3395	5′ GGC GAC TGG GAC AGC A 3′
	3396	5′ CCT GTC TAC TTG CCA CAC 3′
vpr	3397	5′ CGG CGA CTG AAT CTG CTA T 3′
	3398	5′ CCT AAC ACT AGG CAA AGG TG 3′
Exon 3 inclusion	3397	5′ CGG CGA CTG AAT CTG CTA T 3′
	3636	5′ CCG CTT CTT CCT TGT TAT GTC 3′
Exon 3 exclusion	3629	5′ GGC GGC GAC TGG AAG AAG C 3′
	3637	5′ GAG AAG CTT GAT GAG TCT GAC 3′
GH1a	1224	5′ TCT TCC AGC CTC CCA TCA GCG TTT GG 3′
	1225	5′ CAA CAG AAA TCC AAC CTA GAG CTG CT 3′

^aTransfection control.