Fig 1.

Expression of N-truncated PAs in plasmid-transfected cells. (A) 293T cells were transfected with a plasmid for the expression of PA vRNA or PA mutant vRNA (pPolI-PA or PA mutants) in combination with expression plasmids encoding WSN-PB1, -PB2, -NP (1 μg each), and -PA (0.2 μg); an expression plasmid, pCAGGS-PA; or N-truncated PAs (1 μg). Forty-eight hours later, total cell lysates were analyzed by Western blotting for the detection of the full-length PA and the N-truncated PAs by using a mixture of anti-PA monoclonal antibodies. (B) Schematic representation of N-truncated forms of PA. N-truncated PAs are translated from the indicated AUG codons at amino acid positions 155 and 182.