Fig 5.

Viral PFU assay. (A) Viral replication ability in NIH 3T3 cells. MCMVE5gfp, MCMVE5gfpdIE3AM, or MCMVE5gfpdIE3AMRQ was used to infect NIH 3T3 cells, and the cells were collected on the days postinfection indicated. After three cycles of freezing and thawing, the cell-medium mixture was centrifuged and the supernatant was used for PFU assays. Each experiment was performed independently three times, and the mean number of PFU was obtained by averaging the numbers from different dilutions. (B) The same as A, but infection was carried out in the NIH 3T3e1 cell line.