Fig 4.

The number of proliferating B cells in the brain. Mononuclear cells isolated from pooled brains of SINV- and PBS-inoculated mice (n = 4) were analyzed for CD19, CD38, and Ki-67 expression. (A) The flow cytometry gating scheme for detecting Ki-67⁺ B cells in the CLNs and brain. The numbers of Ki-67⁺ B cells in the CLNs (B) and brain (C) are shown. The numbers of Ki-67⁺ or Ki-67⁻B cells expressing intracellular cIgG within the gated CD19⁺ population in the CLNs (D) and brain (E) are plotted. Staining of cells was done in duplicate for each time point. Each point is the mean ± SEM of one experiment.