Fig 6.

The life span of infiltrated B cells in the brain. Mononuclear cells isolated from pooled brains of SINV- and PBS-inoculated mice (n = 4) were analyzed for CD19, CD38, and BrdU expression. (A) The flow cytometry gating scheme for detecting BrdU⁺ B cells in the CLNs and brain 1 day after labeling. Labeling periods were days 4 to 7, days 7 to 10, and days 11 to 14. The numbers of BrdU⁺ B cells above baseline for CLNs (B, C, and D) and brain (E, F, and G) within the gated CD19⁺ population are plotted. Staining of cells was done in duplicate for each time point. The mean PBS value (background labeling) was subtracted from each corresponding SINV time point. Each point is the mean ± SEM of one experiment.