Fig 5.

Effects of peptides (50 μg/ml) on adhesion (a), nuclear transport of VP16 (b), and expression of the ICP27 gene (c). Heparin (100 μg/ml), acyclovir (100 μg/ml), and PBS (pH 7.4) were used as controls. (a) CaSki cells were cooled to 4°C and exposed to HSV-2 preincubated with peptide for 2 h at 37°C (MOI, 1 PFU/cell) and then maintained at 4°C for 3 h. Bound virus was detected by analyzing Western blots of cell lysates for gD. (b) CaSki cells were cooled to 4°C and inoculated with virus (1 PFU/cell) at 4°C for 3 h. The cultures were washed with PBS to remove the unbound viruses and incubated with peptides at 37°C for 3 h. Then, nuclear extracts were eluted and analyzed for nuclear VP16. (c) CaSki cells with synchronized infectivity and citrate buffer treatment were incubated with peptides for 5 h and cultured in fresh medium supplemented with 2% heat-inactivated FBS. Cell lysates were prepared 24 h after entry of virus. Expression of ICP27 was determined by Western blotting.