Fig 8.

Quantification of inhibiting activity of HD5 and E21R-HD5 against strain HIV_HTLV-IIIB. JLTRG cells (1 × 10⁵) grown in 24-well plates were preincubated with 2-fold serially diluted HD5 or E21R-HD5 peptide (0.3906 to 50 μg/ml) or positive-control peptide C34 (0.00064 to 3.2 μg/ml) for 1 h. H9/HIV_HTLV-IIIB cells (1 × 10⁴) were then added to initiate viral infection, and the coculture was maintained for 3 days. Thereafter, the cell samples were analyzed for EGFP expression using flow cytometry. Data presented are means of two independent experiments, with triplicate samples for each point (C34 [a], HD5, and E21R-HD5 [c]). Percent inhibition was calculated by using the formula 100 × [1 − (net percentage of cells EGFP⁺ × mean fluorescence intensity value of cells EGFP⁺ in test group)/that in mock group].