Fig 2.

Effect of PARP-1 on the HIV-1 life cycle. DT40 WT, KO, and h-1 cells were infected with TRIPluc and analyzed 24 h (a) or 10 days (b and c) later. Total HIV cDNA (gag DNA), 2LTR circle DNA, and histone H4 DNA were determined by real-time PCR. Total HIV cDNA and 2LTR circle DNA levels were normalized to the levels of histone H4. The standard deviations represent the variability of triplicate real-time measurements of samples from two (a) or three (c) independent infection experiments. (b) Susceptibility of DT40-derived cells to HIV-1 infection. Infectivity (cell viability-normalized luciferase levels) was determined at 10 days postinfection. Fold infectivity is expressed relative to the infectivity of WT cells. The standard deviations represent the variability of triplicate measurements of one of the infection experiments considered in panel c.