Fig 8.

Restricted incorporation of the H protein into the WT virus particle. (A) Uptake of the H and M proteins. Vero/hSLAM cells were inoculated with the WT or F50P virus at an MOI of 0.01, and at 12 h p.i., the culture medium was replaced by medium including Cyt-D or DMSO. At 72 h p.i., virus particles in the culture medium were collected as pellets by centrifugation passing through a 20% sucrose layer (virus particles), and the cells were solubilized with detergent solution (cells). The M and H proteins as well as actin in the samples were analyzed by immunoblotting with the same antibodies described in the legend for Fig. 7B. (B) Amount of the genome RNA packaged in MV particles. cDNA of virus genome RNA was prepared by reverse transcription using the primer annealing the leader sequence, and then the N gene was amplified by PCR as described in Materials and Methods. □, WT virus without Cyt-D; ○, F50P virus without Cyt-D; ■, WT virus with Cyt-D; ●, F50P virus with Cyt-D.