Fig 6.

Components of the SUMO and DNA repair pathways are recruited to sites associated with HSV-1 genomes in triple-depleted cells. HFs were transduced with either a lentivirus expressing shRNAs against hDaxx, PML, and Sp100 (shDPS) or a scrambled shRNA with no cellular target (shneg). Cells were infected with ICP0-null HSV-1 expressing enhanced yellow fluorescent protein-tagged ICP4 at an MOI of 0.1, fixed at 24 h postinfection, and then simultaneously stained with mouse anti-PML MAb 5E10 and SUMO-1 rabbit serum ab32058, SUMO-2/3 rabbit serum ab3742, or 53BP1 rabbit serum ab21083. Secondary antibodies were Alexa Fluor 555 donkey anti-mouse and Alexa Fluor 647 donkey anti-rabbit antibodies. PML served as a marker for depletion. Bar, 10 μm.