Fig 1.

Construction of the recombinant viruses. Left panel, schematic maps of the EBV genome fragments that encompass four EBV genes presumably involved in virus maturation, before and after recombination with targeting vectors. Recombination results in an exchange between the targeted genes and the kanamycin (kana) resistance cassette. Schematic maps of targeted EBV gene regions around BFLF1/BFRF1A (A), around BBRF1 (B), and around BGRF1/BDRF1 (C). Promoters (arrows), enzyme restriction sites, and the predicted sizes of the resulting fragments after cleavage with BamHI or HindIII are indicated. Right panel, results of the restriction fragment analysis of the various mutant genomes after construction in E. coli or after rescue from the producer cell lines are presented. The results of this analysis concur with the predicted restriction pattern. EBV-WT, Epstein-Barr virus wild type.