Fig 2.

Replicating VEE PIV genomes produce infectious virus very inefficiently, and wt capsid protein can trans-complement the defect in genome packaging. (A) Schematic representations of the viral, PIV, and helper genomes. (B) Release of infectious virus particles from cells electroporated with in vitro-synthesized viral and PIV genomic RNAs (∼4 μg) or coelectroporated with PIV and helper RNAs (∼4 μg of each). Media were harvested at the indicated times, and infectious titers were determined as described in Materials and Methods.