Fig 7.
VEE PIVs are potent inducers of IFN-β. (A) Schematic representations of viral, replicon, and PIV genomes used in the experiment. (B) NIH 3T3 cells (5 × 105) in 6-well Costar plates were infected with the indicated viruses or packaged replicon and PIV genomes at an MOI of 20 infectious units/cell. Media were harvested at 20 h postinfection, and concentrations of released IFN-β were determined as described in Materials and Methods. “VEEV/CRK4−/GFP P1” indicates infection of cells with genome-free particles released from VEEV/CRK4−/GFP-infected cells (see the text for details).