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. 2013 Feb;87(4):2215–2225. doi: 10.1128/JVI.02344-12

Fig 4.

Fig 4

Detection of specificity and subtypes of MAb HA-7 by ELISA. (A) Reactivity of total IgG of HA-7 with fusion proteins containing full-length HA1 (residues 13 to 325 of H5 numbering [21, 25, 46], corresponding to HA1 residues +3 to 322 [30]) and truncated fragments of HA1 of A/Anhui/1/2005(H5N1) virus. ELISA plates were respectively coated with recombinant HA1 proteins fused with human Fc (HA1-Fc), Fd sequence (HA1-Fd), or Fd plus Fc (HA1-Fdc) and HA1 protein without Fd and Fc (HA1-His). Recombinant hIgG1-Fc2 protein (rFc), commercial human IgG Fc protein (IgG-Fc), Fd fused with HIV-1 gp41 (HIV-Fd), and SARS-CoV RBD protein were used as the controls. (B) Reactivity of total IgG of MAb HA-7 with inactivated IAVs containing H5N1, S-OIV H1N1, and seasonal influenza viruses (IAVs). (C) Detection of IgG subtypes of MAb HA-7 using recombinant HA1-His protein as the coating antigen. The data are presented as the mean absorbance at 450 nm (A450) ± standard deviations of data from duplicate wells of MAbs at a dilution of 1:3,200. Panels A to C show data from three independent experiments. (D) Amino acid sequences for HA-7 Fab heavy (VH) and light (VL) chains. CDR1, CDR2, and CDR3 are labeled.