Fig 5.

Epitope mapping and detection of reactivity of MAb HA-7 with denatured recombinant proteins by ELISA. (A) ELISA plates were coated with truncated recombinant HA1 protein fragments covering the full-length HA1 region, followed by the addition of MAb HA-7 to detect the reactivity of total IgG. The data are presented as mean A₄₅₀ values ± standard deviations of data from duplicate wells of MAbs at a dilution of 1:3,200. Shown are representative data from three independent repeat experiments. (B) Reactivity of MAb HA-7 with DTT-denatured recombinant HA1 protein fragments, using corresponding proteins without DTT treatment as a comparison. The data are presented as mean A₄₅₀ values ± standard deviations of data from duplicate wells of MAb at a dilution of 1:51,200.