Fig 3.
RT-QuIC detection of PrPC converting activity in the brain (medulla) of DeerCWD- and FelCWD-inoculated cats. Reaction mixtures were seeded, in duplicate, with cat or deer brain homogenate at a dilution of 10−4. Each data point represents the average ThT fluorescence of each duplicate reaction set. (A) Detection of PrPC converting activity in 2 of 5 primary passage DeerCWD i.c.-inoculated cats (cats 1 and 4). (B) Detection of PrPC converting activity in 5 of 5 secondary passage FelCWD i.c.-inoculated cats. Panels A and B demonstrate detection of PrPC converting activity in CWD+ deer (red dotted lines with X) and lack of converting activity in CWD-negative deer (black circles) and cat (black diamonds) controls.