Fig 5.

siRNA knockdown of Daxx, HDAC1, and DNMT3A and -3B leads to reactivation of reporter gene expression in long-term ASV-GFP-silent populations. (A) Results of siRNA treatment of a population of HeLa cells that contain a silent ASV-GFP provirus with the GFP gene under the control of an internal CMV promoter (HeLa TI-C). (B) Results of siRNA treatment of a population of HeLa cells that contain a silent ASV-GFP provirus with GFP reporter gene expression regulated by the proviral LTR (HeLa TI-L). In panels A and B, GFP expression was determined by FACS 96 h after siRNA addition. NT, not transfected; Mock, mock treated (treated with transfection reagent without siRNA). (C) Assessment of knockdown efficiencies by immunoblotting. Samples were analyzed 96 h after siRNA treatment. GAPDH was used as a loading control.