Table 4.

Comparison of human PSC dissociation protocols.

Method	Dispase	TrypLE/Accutase	EDTA
Cell Survival	Good	Poor	Good
ROCK inhibitor treatment	No	Essential	Not necessary
Rinsing or neutralization of the enzymes	Yes	Yes	No
Centrifugation	No	Yes	No
Time needed for one six-well plate	7–10 min	10–15 min	5–6 min
Damage to extracellular matrix or membrane proteins	Yes	Yes	No
Dependency on different plate coating materialss14	Not Applicable	High	Low
Accessible to transfection	Less Efficient	Efficient	Efficient
Differentiation	Less sensitive	Sensitive	Sensitive
Cryopreservation	Good19	Good with ROCK inhibitors20	Good, ROCK inhibitor optional
Stem cell enrichment	No	No	Yes
Batch Consistency	Poor	Good	Good
Teratoma formation	Good	Poor	Good
Source	Commercial	Commercial	Homemade
Cost	High	High	Low