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. Author manuscript; available in PMC: 2013 Feb 13.
Published in final edited form as: Vaccine. 2009 Aug 3;27(42):5875–5884. doi: 10.1016/j.vaccine.2009.07.060

Fig. 4.

Fig. 4

Comparative TLR5 activity of VN vaccine constructs. Each of the VN HA vaccines was tested for TLR5 activity using HEK-293 cells. Vaccines were compared to flagellin alone (STF2.6xHis) at the indicated doses ranging from 40,000 to 0.01 ng/ml. HEK-293 cells were incubated with vaccine constructs for 20 h and supernatant was tested for IL-8 using a specific antibody pair (BD). Data is shown as the mean ± standard deviation of IL-8 (pg/ml).