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Effect of various primuline concentrations on a HCV helicase-catalyzed MBHA. (A) MBHAs were performed with the indicated concentration of the known helicase inhibitor primuline. Solid lines show nonlinear regression fits described in text. (B) Secondary plot of initial velocities (triangles) and F0s (circles) fit to a dose response equation as described in text. Dotted line indicates the IC50 value determined by nonlinear regression.
