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. Author manuscript; available in PMC: 2013 Feb 13.
Published in final edited form as: Methods Enzymol. 2012;511:463–483. doi: 10.1016/B978-0-12-396546-2.00021-8

Figure 21.3.

Figure 21.3

Evaluation of alternate fluorophores for use in MBHA-based screens. Each substrate was labeled with Iowa Black RQ (IAbRQ) and one of the four indicated fluorophores available from Integrated DNA technologies (Coralville, IA). Each panel shows results from a single 96-well low-volume plate containing 8 reactions without DNA (BKG), 44 reactions with 2% DMSO, and 44 reactions containing the HCV helicase inhibitor primuline at a final concentration of 50 µM in 2% DMSO. Solid lines show means of positive and negative controls and the dotted lines show three times the standard deviations.