Figure 5.
Kiss1 mRNA expression in POA from oil- and E2-treated females. Matched film images of Kiss1 mRNA expression in the PVpo (periventricular POA) in 24-hour and 42-hour ovariectomized oil (A,C)- and E2 (B,D)-treated animals. The darkness of the image represents the density of mRNA expression. For abbreviations see list. E: Quantitative analysis of Kiss1 mRNA in the PVpo from oil-and E2-treated females at 24 hours (negative feedback) and 42 hours (positive feedback); n = 5–6 animals (two-tailed Student’s t-test, **P < 0.01, ***P < 0.001). Scale bar = 1 mm.