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. Author manuscript; available in PMC: 2014 Feb 6.
Published in final edited form as: J Am Chem Soc. 2013 Jan 18;135(5):1749–1759. doi: 10.1021/ja307710d

Figure 1.

Figure 1

Representative results of the ATP/PPi exchange assay for L-pHPG activating modules A1T1-His6, A3T3 E3C4-His6 and C5A5T5TE-His6 and L-serine activating module, A4T4-His6 at 0.5uM. For modules A1T1-His6 and A4T4-His6 which require addition of a MbtH protein for activity, His6-NocI was added at 2 uM final concentration. All assays were performed at room temperature.