Table 1.
Oligonucleotide primers used for NRPS expression vector construction.
| Description | Label | Primer Sequence (5′ to 3′) |
|---|---|---|
| A1T1 | M1 For (Nco I) | GCGCCATGGGGGACAGCGAGGAGTGGGAGCGC |
| M1 Rev (Xho I) | GCGCTCGAGGGCCAGCTCCTCGATCCGCCTGGCCAG | |
| A2T2 | M2 For (Nco I) | GCCCATGGTTAGCACCGGCGCGCCGGTCGAACC |
| M2 Rev (BamH I) | GCGGATCCCGCGTCGTCGGCCCACGGC | |
| A3T3 E3C4 | M3 For (Nco I) | TCTTCCATGGTGCTCGGGGACGAC |
| M3 Rev (BamH I) | TATAGGATCCCGGGTTCCTTCTCCCCTCG | |
| A4T4 | M4 For (Nco I) | GCCCCATGGCCTTTCGAGTGCGCGACCTGTTCGCCG |
| M4 Rev (Xho I) | GGCCTCGAGGGTGGACGCCTCGTCGGCG | |
| C5A5T5TE | M5 For (Nco I) | GCGCTCCATGGACGAGGAGGCGCTGCTGGCG |
| M5 Rev | TATAAAGCTTCCGCTCTCCTCCCAGCGCGC |