Table 2.
Oligonucleotide primers used for NocI and NocP expression vector construction.
| Vector | Label | Primer Sequence (5′ to 3′) |
|---|---|---|
| pCDFDuet | NocI For (Nco I) | GCCCCATGGTGGGAGAGAACGAGGATTCCGG |
| NocI Rev (Hind III) | GCGAAGCTTTCAAGCCCGGTCCCCGGCC | |
| pTYB12 | NocI For (Bsm I) | GCCGAATGCTCTGGGAGAGAACGAGGATTCCGG |
| NocI Rev (Xho I) | GCGCTCGAGTCAAGCCCGGTCCCCGGCC | |
| pET28b(+) | NocP For (Nde I) | GCGGCATATGAACCCCTTCGACGACCACGACCGCGCT |
| NocP Rev (Xho I) | GCGCTCGAGTCAGGCGTCGGGGTCGGGCGCGCCCGCGCG | |
| pCDFDuet | NocP For (Nde I) | GCGGCATATGAACCCCTTCGACGACCACGACCGCGCT |
| NocP Rev (Xho I) | GCGCTCGAGTCAGGCGTCGGGGTCGGGCGCGCCCGCGCG |