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. Author manuscript; available in PMC: 2014 Feb 12.
Published in final edited form as: Biochemistry. 2013 Jan 31;52(6):1037–1044. doi: 10.1021/bi301393v

Figure 7.

Figure 7

Relative fluorescence intensities of delipidated ApolPBP1 mutants upon addition of 5 μM AMA to 1 μM protein samples. Light grey: pH 6.5; Dark grey: pH 4.5. Error bars indicate standard deviation of the data. ApolPBP1wt and ApolPBP1H70A/H95A show similar binding at pH 6.5. ApolPBP1wt has negligible binding at pH 4.5 while ApolPBP1H70A/H95A shows considerable binding at same pH. Both ApolPBP1ΔP129-V142 and ApolPBP1H70A/H95AΔP129-V142 show reduced binding (23–28 % of the wild type protein at pH 6.5) at both pH 6.5 and 4.5.